OUYANG Yu (欧阳雨)
(The Experimental and Managing Center, Linyi University, Linyi 276005, China)
Abstract：Raman spectra and surface-enhanced Raman scattering (SERS) spectra of filter paper and silica gel plate are obtained at 785-nm excitation wavelength. The results show that filter paper has thirteen Raman lines in 800-1 500 cm-1 and silica gel plate has eight lines in the region of 400-1 200 cm-1. The frequencies and Raman active modes are assigned. By comparing their Raman spectra and SERS spectra, we find that the silver colloid could repress the Raman intensities of filter paper and silica gel plate, and reduce their effects on the combination of thin-layer chromatographic (TLC) and SERS. According to SERS spectra, the bands at 997 cm-1,1 094 cm-1,1 118 cm-1,1 336 cm-1 and 1 378 cm-1 for filter paper and the peak of ν1[SO4] symmetric stretching vibration (≈1 017 cm-1) for silica gel plate also have high intensities, and those lines should be paid enough attention in TLC-SERS.
Key words：chromatography; paper chromatography; silica gel plate; Raman spectroscopy; Surface-enhenced Raman scattering(SERS)
CLD number： O657.37 Document code： A
Article ID： 1674-8042(2012)04-0313-04 doi： 10.3969/j.issn.1674-8042.2012.04.002
References
[1] Sequaris J M, Koglin E. Direct analysis of high-performance thin-layer chromatography spots of nucleic purine derivatives by surface-enhanced Raman scattering spectrometry. Analytical Chemistry, 1987, 59(3): 525-527.
[2] REN Bing, TIAN Zhong-qun. The progress in surface-enhanced Raman spectroscopy. Modern Instruments, 2004, 5(9): 1-13.
[3] WANG Yuan, GUO Zhan-sheng, WANG Ying-feng, et al. In situ thin layer chromatography Fourier transform surface enhanced Raman spectrum study on ingredients of berberine. Spectroscopy and Spectral Analysis, 2002, 22(5)： 745-748.
[4] TIAN Zhong-qun, REN Bing. Adsorption and reaction at electrochemical interfaces as probed by surface-enhanced Raman spectroscopy. Annual Review of Physical Chemistry, 2004, 55: 197-229.
[5] CAO Xiao-wei. Study of elecorode potential effect on acid-base behavior of ω-functionalized self-assembled monolayers using Fourier transform surface-enhanced Raman scattering spectroscopy. Journal of Raman Spectroscopy, 2005, 36(3): 250-256.
[6] István K, Keresztury G, Szép A. Normal Raman and surface enhanced Raman spectroscopic experiments with thin layer chromatography spots of essential amino acids using different laser excitation sources. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2003, 59(8): 1709-1723.
[7] WANG Yuan , ZHANG Jin-zhi, MA Xin-yong. TLC-FT-SERS study on a pair optic isomer in ephedra. Spectroscopy and Spectral Analysis, 2004, 24(11): 1373-1375.
[8] Brosseau C L, Gambardella A, Casadio F, et al. Chromatography-surface enhanced Raman spectroscopy and in situ on the fiber analysis. Analytical Chemistry, 2009, 81(8): 3056-3062.
[9] LIU Yong-liang, Kokot S, Sambi T J. Vibrational spectroscopic investigation of Australian cotton cellulose fibres Part 1. A Fourier transform Raman study. Analyst, 1998, 4: 123, 633-636.
[10] Knittle E, Phillips W, Williams Q. Infrared and Raman spectroscopic study of gypsum at high pressures. Physics and Chemistry of Minerals, 2001, 28(9): 630- 634.
[11] Sarma L P, Prasad P S R, Ravikumar N. Raman spectroscopic study of phase transitions in natural gypsum. Journal of Raman Spectroscopy, 1998, 29(9): 851-856.
