ZHONG Yan-mei1, FENG Yi-fan1, TAN Yu-zhi2
(1. Central Laboratory, Guangdong Pharmaceutical University, Guangzhou 510006, China;2. Department of Pharmacology, Guangdong Pharmaceutical University, Guangzhou 510006, China)
Abstract： To establish a rapid and effective method for analysis and identification of the alkaloids from Ranunculus japonicus Thunb by ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) and discuss their fragmentation regularity, the UPLC/Q-TOF-MS was used to identify the alkaloids from Ranunculus japonicus Thunb by their MS data, tandem characteristic fragment ions and standards. In the end, 12 alkaloids were identified from Ranunculus japonicus for the first time, and their fragmentation regularity was discussed. Thus, a rapid and effective analysis and identification method for the alkaloids from Ranunculus japonicus by UPLC/Q-TOF-MS is established.
Key words： alkaloids; ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry (UPLC/Q-TOF-MS); Ranunculus japonicas
CLD number： R284.1 Document code： A
Article ID： 1674-8042(2014)03-0087-07 doi： 10.3969/j.issn.1674-8042.2014.03.017
References
[1] CHEN Jun-hui, WANG Feng-mei, LIU Jie, et al. Analysis of alkaloids in Coptis chinensis Franch by acceleratedsolvent extraction combined with ultraperformance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections. Analytica Chimica Acta, 2008, 613(2): 184-195.
[2] CHEN Jun-hui, ZHAO Heng-qiang, WANG Xiao-ru, et al. Analysis of major alkaloids in Rhizoma coptidis by capillary elec tro phoresis-electrospray-time of flightmass spectrometry with different background el ectrolytes. Electrophoresis, 2008, 29(10) : 2135-2147.
[3] ZHANG Jing, YU Jin, DONG Jun, et al. Systematic screening and characterization of tertiary andquaternary alkaloids from corydalis yanhusuo W.T. Wang using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. Talanta, 2009, 78(2): 513-522.
[4] LIU Meng-hua, TONG Xin, WANG Jin-xu, et al. Rapid separation and identification of multiple constituents in traditional Chinese medicine formula Shenqi Fuzheng Injection by ultra-fast liquid chromatography combined with quadrupole-time-of-flight mass spectrometry.Journal of Pharmaceutical and Biomedical Analysis, 2013, 74: 141-155.
[5] RUI Wen, ZHONG Yan-mei, FENG Yi-fan, et.al. Rapid analysis of the main components in the total glycosides of Ranunculus japonicus by UPLC/Q-TOF-MS. Natural Product Communications, 2010, 5(5): 795-800.
[6] WU Shi-hua, LIANG Jun-ling. Counter-current chromatography for high throughput analysis of natural products. Combinatorial Chemistry & High Throughput Screening, 2010, 13(10): 932-942.
[7] Dostl J, Man S, Sek′ov P, et al. Berberine and coptisine free bases. Journal of Molecular Structure, 2004, 687(1/2/3), 135-142.
[8] XIE Zong-wan. Compilation of the national Chinese herbal medicine. Beijing: People's Health Publishing House, 1996: 201.
[9] CAI Sa, TAN Yu-zhi, LI Shu-fang, et al. Effects of Ranunculus japonicus Thumb Extractant on uterine smooth muscle and its mechanism.Academic. Journal of Guangdong College Pharmacy, 2004, 20(1): 31-39.
[10] DENG Ya-ting, LIAO Qiong-feng, LI Su-hua, et al. Simultaneous determination of berberine, palmatine and jatrorrhizine by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of coptis-evodia herb couple. Journal of Chromatograph B, 2008, 863(2): 195-205.
[11] YU Sen, PANG Xiao-yan, DENG Yuan-xiong, et al. A sensitive and specific liquid chromatography mass spectrometry method for simultaneous determination of berberine, palmatine, coptisine, epiberberine and jatrorrhizine from Coptidis Rhizoma in rat plasma. International Journal of Mass Spectrometry, 2007, 268(1): 30-37.
[12] WANG Dao-wu, LIU Zhi-qiang, GUO Ming-quan, et al. Structural elucidaton and identification of alkaloids in Rhizoma Coptidis by electrospray ionization tandem mass spectrometry. Journal of Mass Sectrometry, 2004, 39(11): 1356-1365.
[13] Deevanhxay P, Suzuki M, Maeshibu N, et al. Simultaneous characterization of quaternary alkaloids, 8-oxoprotoberberine alkaloids, and a steroid compound in Coscinium fenestratum by liquid chromatography hybrid ion trap time-of-flight mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 2009, 50(3): 413-425.
[14] WU Wei, LIANG Zhi-tao, ZHAO Zhong-zhen, et al. Direct analysis of alkaloid profiling in plant tissue by using matrix-assisted laser desorption/ionizationmass spectrometry. Journal of Mass Spectrometry, 2007, 42(1): 58-69
[15] Min Y D, Yang M C, Lee K H, et al. Protoberberine alkaloids and their reversal activity of P-gp expressed multidrug resistance(MDR) from the Rhizome of Coptis japonica Makino. Archives of Pharmacal Research, 2006, 29(9): 757-761.
[16] ZHANG Yu-feng, SHI Qi-rong, SHI Pei-ying, et al. Characterization of isoquinoline alkaloids, diterpenoids and steroids in the Chinese herb Jin-Guo-Lan (Tinospora sagittata and Tinospora capillipes) by high-performance liquid chromatography/electrospray ionization with multistage mass spectrometry. Rapid Communications in Mass Spectrometry, 2006, 20(15): 2328-2342.
[17] MA Hong-da, WANG Yong-jun, GUO Tao, et al. Simultaneous determination of tetrahydropalmatine, protopine, and palmatine in rat plasma by LC-ESI-MS and its application to a pharmacokinetic study. Journal of Pharmaceutical and Biomedical Analysis, 2009, 49(2): 440-446.
[18] HU Yong-mei, SU Guang-hai, Sze S C, et al. Quality assessment of Cortex Phellodendri by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry. Biomedical. Chromatography, 2010, 24(4): 438-453.
[19] WU Wu-nan, HUANG Chong-heng. Structural elucidation of isoquinoline, isoquinolone, enzylisoquinoline, aporphine, and phenanthrene alkaloids using API-ionspray tandem mass spectrometry. The Chinese Pharmaceutical Journal, 2006, 58(1): 41-55.
基于UPLC/Q-TOF-MS技术的毛茛生物碱类成分快速分析鉴定
钟艳梅1，冯毅凡1，谭毓治2
(1. 广东药学院 中心实验室， 广东 广州 510006； 2. 广东药学院 药理学教研室， 广东 广州 510006)
摘要:应用液质联用技术UPLC/Q-TOF-MS建立一种有效快速分析鉴定毛茛生物碱类成分的方法并研究其质谱裂解规律。 采用液质联用技术UPLC/Q-TOF-MS， 根据毛茛生物碱类成分的一级质谱数据， 二级特征裂解碎片及标准品比对鉴定毛茛生物碱类成分。 共鉴定了12种生物碱类成分， 并探讨了其裂解规律。 首次从毛茛中分离鉴定了12种生物碱类物质， 建立了一种有效的基于UPLC/Q-TOF-MS鉴定毛茛生物碱类成分的方法。
关键词:生物碱；超高效液相色谱-四级杆-飞行时间串联质谱技术 (UPLC/Q-TOF-MS)； 毛茛
引用格式：ZHONG Yan-mei, FENG Yi-fan, TAN Yu-zhi. Rapid analysis and identification for the alkaloids from Ranunculus japonicus by UPLC/Q-TOF-MS. Journal of Measurement Science and Instrumentation, 2014, 5(3): 87-93. [doi: 10.3969/j.issn.1674-8042.2014.03.017]
[full text view]
