Alaa-Eldin A Abd-Elmageed, Essam M Elmoghazy, Fatma Elsharkawy
（Photometry and Radiometry division, National Institute for Standards (NIS), Giza, 12211, Egypt）
Abstract： Ultraviolet-visible (UV-Vis) spectrophotometry is commonly used in analytical laboratories for qualitative and quantitative analyses. To make the data obtained reliable, an experimental instrument must be calibrated. National Institute for Standards (NIS) in Egypt builds up a photometric method to ensure the competence of absorbance and transmittance to the standard international and national requirements. This paper presents an instrument for UV-Vis measurement, discusses the factors affecting measurement reliability and estabishes the uncertainty model including correspoding measurement parameters.
Key words： spectrophotometer; standardization; absorbance; uncertainty
CLD number： TH744.12+2 Document code： A
Article ID： 1674-8042（2016）01-0040-04 doi： 10.3969/j.issn.1674-8042.2016.01.008
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紫外可见光谱区分光光度计的标准化
Alaa-Eldin A Abd-Elmageed, Essam M Elmoghazy, Fatma Elsharkawy
（Photometry and Radiometry division, National Institute for Standards (NIS), Giza, 12211, Egypt）
摘 要: 紫外可见光谱测量通常用于实验室的定性和定量分析。 为确保测量值可靠， 实验仪器必须经过校准。 埃及国家标准化所建立了一种光度测定法， 其吸收和传输能力满足国际和国家要求。 本文介绍了紫外可见光谱测及测量仪， 讨论了影响测量可靠性的因素， 并讨论了包含相关测量参数的不确定度模型。
关键词: 分光光度计； 标准化； 吸收； 不确定度
引用格式： Abd-Elmageed A-E A, Elmoghazy E M, Fatma Elsharkawy. Spectrophotometer standardization in UV-Vis spectral region. Journal of Measurement Science and Instrumentation, 2016, 7(1)： 40-43. ［doi： 10.3969／j.issn.1674-8042.2016.01.008］
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